Diagnosis and identification ofLeishmaniaspp. from Giemsa-stained slides, by real-time PCR and melting curve analysis in south-west of Iran
نویسندگان
چکیده
منابع مشابه
Diagnosis and Characterization of Leishmania Species in Giemsa-Stained slides by PCR-RFLP
Background: Direct identification of Leishmania species in Giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. This study was designed to isolate Leishmani spp. from Giemsa-stained smears and to characterize them by PCR technique. Methods: A total of 48 Giemsa-Stained slides from confirmed cases of leishmaniasis were examined under a ligh...
متن کاملdiagnosis and characterization of leishmania species in giemsa-stained slides by pcr-rflp
background: direct identification of leishmania species in giemsa-stained slides without parasite culturing in the areas where multiple species exist, is very helpful. this study was designed to isolate leishmani spp. from giemsa-stained smears and to characterize them by pcr technique. methods: a total of 48 giemsa-stained slides from confirmed cases of leishmaniasis were examined under a ligh...
متن کاملIdentification of Leishmania Species Using PCR Assay on Giemsa-Stained Slides Prepared From Cutaneous Leishmaniasis Patients
BACKGROUND Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. The slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملsimultaneous detection and identification of hbv and htlv-i viruses by melting curve analysis ofmultiplex real-time pcr
background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...
متن کاملRapid, sensitive, and discriminating identification of Naegleria spp. by real-time PCR and melting-curve analysis.
The free-living amoeboflagellate genus Naegleria includes one pathogenic and two potentially pathogenic species (Naegleria fowleri, Naegleria italica, and Naegleria australiensis) plus numerous benign organisms. Monitoring of bathing water, water supplies, and cooling systems for these pathogens requires a timely and reliable method for identification, but current DNA sequence-based methods ide...
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ژورنال
عنوان ژورنال: Annals of Tropical Medicine & Parasitology
سال: 2011
ISSN: 0003-4983,1364-8594
DOI: 10.1179/2047773211y.0000000014